Forward Genetics in Toxoplasma gondii Reveals a Family of Rhoptry Kinases That Mediates Pathogenesis
نویسندگان
چکیده
منابع مشابه
Novel structural and regulatory features of rhoptry secretory kinases in Toxoplasma gondii.
Serine/threonine kinases secreted from rhoptry organelles constitute important virulence factors of Toxoplasma gondii. Rhoptry kinases are highly divergent and their structures and regulatory mechanism are hitherto unknown. Here, we report the X-ray crystal structures of two related pseudokinases named ROP2 and ROP8, which differ primarily in their substrate-binding site. ROP kinases contain a ...
متن کاملCloning rhoptry protein 1 (ROP1) gene of Toxoplasma gondii (RH) in expression vector
Toxoplasma gondii contain various immunogenic antigens. The most important Toxoplasma antigens are somatic and excreted/secreted antigens. Rhoptry proteins are known as excreted/secreted antigens. These antigens have been proposed as a vaccine candidate against toxoplasmosis. The main objective of the present work was cloning rhoptry protein1 (ROP1) Gene of Toxoplasma gondii (RH) in a cloning...
متن کاملThe Toxoplasma gondii Rhoptry Kinome Is Essential for Chronic Infection
UNLABELLED Ingestion of the obligate intracellular protozoan parasite Toxoplasma gondii causes an acute infection that leads to chronic infection of the host. To facilitate the acute phase of the infection, T. gondii manipulates the host response by secreting rhoptry organelle proteins (ROPs) into host cells during its invasion. A few key ROP proteins with signatures of kinases or pseudokinases...
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The simultaneous targeting of host and pathogen processes represents an untapped approach for the treatment of intracellular infections. Hypoxia-inducible factor-1 (HIF-1) is a host cell transcription factor that is activated by and required for the growth of the intracellular protozoan parasite Toxoplasma gondii at physiological oxygen levels. Parasite activation of HIF-1 is blocked by inhibit...
متن کاملEvaluating the serological applications of Toxoplasma gondii rhoptry protein 1 (ROP1) antigen
Introduction: Toxoplasma gondii (T. gondii) is distributed worldwide and infects most species. The serious incidence and severe or fatal injury caused by T. gondii infection clearly indicates the necessity for the event of a vaccine. The current study goals were to evaluate serological applications of Toxoplasma gondiirhoptry protein 1 (ROP1) ...
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ژورنال
عنوان ژورنال: Eukaryotic Cell
سال: 2009
ISSN: 1535-9778,1535-9786
DOI: 10.1128/ec.00107-09